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Thermo Fisher raw dna sequence reads
Raw Dna Sequence Reads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Molecular Devices LLC raw luminescence intensity data
Raw Luminescence Intensity Data, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC data raw proximity proteomic data
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Biowest SAS dulbecco's modified eagle's medium (dmem) growth media
Dulbecco's Modified Eagle's Medium (Dmem) Growth Media, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC raw264 7 murine macrophage cell line
Raw264 7 Murine Macrophage Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioResource International Inc raw 264.7 mouse macrophage cell line
Raw 264.7 Mouse Macrophage Cell Line, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BPS Bioscience raw 264 7 cells
DCAC treatment led to increased miR-21 expression in RAW 264.7 cells. (A) Increased expression of miR-21 in the DCAC-treated RAW 264.7 cells and its amelioration by SFN. (B, C) mRNA expression of NF-κB (p65) and IL-12 in RAW 264.7 cells transfected with miR-21 inhibitors. n = 4 in each group. (D) Protein expression of NF-κB (p65) and its densitometry analysis in RAW 264.7 cells following DCAC treatment and transfection with miR-21 inhibitors. n = 3 in each group. *p < 0.05 vs. controls; # p < 0.05 vs. DCAC-treated RAW 264.7 cells. DCAC, dichloroacetyl chloride; SFN, sulforaphane.
Raw 264 7 Cells, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher dna
DCAC treatment led to increased miR-21 expression in RAW 264.7 cells. (A) Increased expression of miR-21 in the DCAC-treated RAW 264.7 cells and its amelioration by SFN. (B, C) mRNA expression of NF-κB (p65) and IL-12 in RAW 264.7 cells transfected with miR-21 inhibitors. n = 4 in each group. (D) Protein expression of NF-κB (p65) and its densitometry analysis in RAW 264.7 cells following DCAC treatment and transfection with miR-21 inhibitors. n = 3 in each group. *p < 0.05 vs. controls; # p < 0.05 vs. DCAC-treated RAW 264.7 cells. DCAC, dichloroacetyl chloride; SFN, sulforaphane.
Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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WuXi AppTec c150 protein
DCAC treatment led to increased miR-21 expression in RAW 264.7 cells. (A) Increased expression of miR-21 in the DCAC-treated RAW 264.7 cells and its amelioration by SFN. (B, C) mRNA expression of NF-κB (p65) and IL-12 in RAW 264.7 cells transfected with miR-21 inhibitors. n = 4 in each group. (D) Protein expression of NF-κB (p65) and its densitometry analysis in RAW 264.7 cells following DCAC treatment and transfection with miR-21 inhibitors. n = 3 in each group. *p < 0.05 vs. controls; # p < 0.05 vs. DCAC-treated RAW 264.7 cells. DCAC, dichloroacetyl chloride; SFN, sulforaphane.
C150 Protein, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Korean Cell Line Bank raw 264 7 cells
DCAC treatment led to increased miR-21 expression in RAW 264.7 cells. (A) Increased expression of miR-21 in the DCAC-treated RAW 264.7 cells and its amelioration by SFN. (B, C) mRNA expression of NF-κB (p65) and IL-12 in RAW 264.7 cells transfected with miR-21 inhibitors. n = 4 in each group. (D) Protein expression of NF-κB (p65) and its densitometry analysis in RAW 264.7 cells following DCAC treatment and transfection with miR-21 inhibitors. n = 3 in each group. *p < 0.05 vs. controls; # p < 0.05 vs. DCAC-treated RAW 264.7 cells. DCAC, dichloroacetyl chloride; SFN, sulforaphane.
Raw 264 7 Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher protein a agarose beads
DCAC treatment led to increased miR-21 expression in RAW 264.7 cells. (A) Increased expression of miR-21 in the DCAC-treated RAW 264.7 cells and its amelioration by SFN. (B, C) mRNA expression of NF-κB (p65) and IL-12 in RAW 264.7 cells transfected with miR-21 inhibitors. n = 4 in each group. (D) Protein expression of NF-κB (p65) and its densitometry analysis in RAW 264.7 cells following DCAC treatment and transfection with miR-21 inhibitors. n = 3 in each group. *p < 0.05 vs. controls; # p < 0.05 vs. DCAC-treated RAW 264.7 cells. DCAC, dichloroacetyl chloride; SFN, sulforaphane.
Protein A Agarose Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DCAC treatment led to increased miR-21 expression in RAW 264.7 cells. (A) Increased expression of miR-21 in the DCAC-treated RAW 264.7 cells and its amelioration by SFN. (B, C) mRNA expression of NF-κB (p65) and IL-12 in RAW 264.7 cells transfected with miR-21 inhibitors. n = 4 in each group. (D) Protein expression of NF-κB (p65) and its densitometry analysis in RAW 264.7 cells following DCAC treatment and transfection with miR-21 inhibitors. n = 3 in each group. *p < 0.05 vs. controls; # p < 0.05 vs. DCAC-treated RAW 264.7 cells. DCAC, dichloroacetyl chloride; SFN, sulforaphane.

Journal: Frontiers in Immunology

Article Title: Differential Expression of miRNAs in Trichloroethene-Mediated Inflammatory/Autoimmune Response and Its Modulation by Sulforaphane: Delineating the Role of miRNA-21 and miRNA-690

doi: 10.3389/fimmu.2022.868539

Figure Lengend Snippet: DCAC treatment led to increased miR-21 expression in RAW 264.7 cells. (A) Increased expression of miR-21 in the DCAC-treated RAW 264.7 cells and its amelioration by SFN. (B, C) mRNA expression of NF-κB (p65) and IL-12 in RAW 264.7 cells transfected with miR-21 inhibitors. n = 4 in each group. (D) Protein expression of NF-κB (p65) and its densitometry analysis in RAW 264.7 cells following DCAC treatment and transfection with miR-21 inhibitors. n = 3 in each group. *p < 0.05 vs. controls; # p < 0.05 vs. DCAC-treated RAW 264.7 cells. DCAC, dichloroacetyl chloride; SFN, sulforaphane.

Article Snippet: RAW 264.7 cells (30,000 cells/100 μl in BPS medium) were plated in 96-well plates and allowed to adhere for 24 h. For transfection, 100 ng of DNA constructs [NF-κB Transient Pack (BPS Biosciences, San Diego, CA, USA) consisting of NF-κB reporter vector + constitutively expressing Renilla luciferase vector or NC reporter (non-inducible luciferase vector + Renilla luciferase vector)] along with 20 nM of miR-690 mimics or respective 20 nM of miRNA control were co-transfected into RAW 264.7 cells using Lipofectamine 2000 (Thermo Fisher Scientific).

Techniques: Expressing, Transfection

DCAC treatment led to increased expression of miR-690 in RAW 264.7 cells. (A) Increased expression of miR-690 in the DCAC-treated RAW 264.7 cells. SFN treatment ameliorated the effect of DCAC. (B, C) mRNA expression of NF-κB (p65) and IL-12 in RAW 264.7 cells transfected with miR-690 mimics. n = 4 in each group. (D) Protein expression of NF-κB (p65) and its densitometry analysis in RAW 264.7 cells. (E) NF-κB-luciferase reporter plasmid along with miR-690 mimics or control were co-transfected into RAW 264.7 cells. (F) Predicted miR-690 targeting region in 3′ UTR of NFkB. Luciferase activity is the ratio of Renilla/ Firefly . Renilla is raw renilla luciferase activity, whereas Firefly is firefly luciferase activity. The 3′ UTR starts at bp 6,577 and ends at bp 6,627. n = 3 in each group. *p < 0.05 vs. controls; # p < 0.05 vs. DCAC-treated RAW 264.7 cells. DCAC, dichloroacetyl chloride; SFN, sulforaphane.

Journal: Frontiers in Immunology

Article Title: Differential Expression of miRNAs in Trichloroethene-Mediated Inflammatory/Autoimmune Response and Its Modulation by Sulforaphane: Delineating the Role of miRNA-21 and miRNA-690

doi: 10.3389/fimmu.2022.868539

Figure Lengend Snippet: DCAC treatment led to increased expression of miR-690 in RAW 264.7 cells. (A) Increased expression of miR-690 in the DCAC-treated RAW 264.7 cells. SFN treatment ameliorated the effect of DCAC. (B, C) mRNA expression of NF-κB (p65) and IL-12 in RAW 264.7 cells transfected with miR-690 mimics. n = 4 in each group. (D) Protein expression of NF-κB (p65) and its densitometry analysis in RAW 264.7 cells. (E) NF-κB-luciferase reporter plasmid along with miR-690 mimics or control were co-transfected into RAW 264.7 cells. (F) Predicted miR-690 targeting region in 3′ UTR of NFkB. Luciferase activity is the ratio of Renilla/ Firefly . Renilla is raw renilla luciferase activity, whereas Firefly is firefly luciferase activity. The 3′ UTR starts at bp 6,577 and ends at bp 6,627. n = 3 in each group. *p < 0.05 vs. controls; # p < 0.05 vs. DCAC-treated RAW 264.7 cells. DCAC, dichloroacetyl chloride; SFN, sulforaphane.

Article Snippet: RAW 264.7 cells (30,000 cells/100 μl in BPS medium) were plated in 96-well plates and allowed to adhere for 24 h. For transfection, 100 ng of DNA constructs [NF-κB Transient Pack (BPS Biosciences, San Diego, CA, USA) consisting of NF-κB reporter vector + constitutively expressing Renilla luciferase vector or NC reporter (non-inducible luciferase vector + Renilla luciferase vector)] along with 20 nM of miR-690 mimics or respective 20 nM of miRNA control were co-transfected into RAW 264.7 cells using Lipofectamine 2000 (Thermo Fisher Scientific).

Techniques: Expressing, Transfection, Luciferase, Plasmid Preparation, Activity Assay